EUROPEAN WORKSHOP ON BACTERIAL PROTEIN TOXINS

June 25 - June 29, 2023, Scientific Programme

All times are British Summer Time BST

Day 1, Sunday PM, June 25, 2023 

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| 15:00 – 17.45 | Arrival, Registration and Check-in,

| 17.45 – 18.00 | Welcome address

| 18.00 – 19.00 | The EMBO Keynote Lecture:  Stefan Raunser, MPI of Molecular Physiology, Germany. Mechanism of Action of Tc Toxins: Protein Translocation Through Bacterial Syringes

| 19.00 | Dinner and informal evening social in the Ballroom

Day 2, Monday AM, June 26, 2023 

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Session 1 - Mechanism of toxin effectors and their secretion systems 
Chair: Laure Journet

EMBO Young Investigator Lecture
| 9.00 – 9.30 | Martin Pilhofer, ETH Zurich, Switzerland. Mechanism and regulation of bacterial predation via ixotrophy

| 9.30 – 9.45 | Jana Kamanova, Czech Academy of Sciences, Czech Republic. Assembly and function of the needle tip filament of the Bordetella type III secretion system
 
| 9.45 – 10.15Alain Filloux, Imperial College London, UK. Pull-down of effectors with Hcp rings and structural details of T6SS onboarding.

| 10.15 – 10.30Somnath Dutta, Indian Institute of Science, India. Deciphering the structural reorganization of bacterial pore forming toxins on lipid bio membranes using single particle Cryo-EM

| 10.30 – 10.45 | Poster mini talks 

Jonathan Pruneda, Oregon Health & Science University, USA

Michal Bejerano-Sagie, Hebrew University of Jerusalem, Israel

Katharina Kubatzky, Heidelberg University, Germany

| 10.45 – 11:15 | Coffee Break

| 11.15 – 11.45 | John Whitney, McMaster University, Canada. New insights into toxin export by Gram-positive bacteria


| 11.45 – 12.00 | Alex Johnson, Harvard Medical School, USA. Cryo-EM structure of an active bacterial gasdermin oligomer

| 12.00 – 12.15 | Benjamin Aroeti, University of Jerusalem, Israel. EspH – The Mechanism of Action

| 12.15 – 12.45 | Marek Basler, Biozentrum Basel, Switzerland. Spatial and temporal regulation of Type 6 Secretion System dynamics during interactions with bacterial and eukaryotic cells

| 12.45 – 13.00 | Poster mini talks 

Panagiotis Papatheodorou, Ulm University Medical Center, Germany

Angélique Perret, Université de Genève, Switzerland

Hoi Ching Cheung, University of Basel, Switzerland

| 13:00 – 14:30 | Lunch

Day 2, Monday PM, June 26, 2023 

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Session 2 - Host Responses and Immunity 
Chair: Karla Satchell

| 14.30 - 15.00  | Charlotte Odendall,  Kings College London, UK. Interferon:Tug of War between Host and Pathogen

| 15.00 – 15.15 | Kim Robinson, Nanyang Technological University, Singapore. Corynebacterium diphtheriae causes ribotoxic stress and NLRP1 inflammasome activation in a model of cutaneous diphtheria
 
| 15.15 – 15.45 | Laurent Boyer, Université Côte d'Azur, France. Innate immune sensing of RhoGTPase-activating bacterial toxins

| 15.45 – 16.00 | Poster mini talks 

Laure Journet, Aix Marseille University, France

Shihui Liu, University of Pittsburgh School of Medicine, USA

Mohamed ElGhazaly, University of Sheffield, UK

| 16.00 – 16.30 | Coffee Break

| 16.30 – 16.45 | Etienne Meunier, University of Toulouse, France. EEF2-inactivating toxins engage the NLRP1 inflammasome and promote epithelial barrier disruption upon Pseudomonas infection   

Lecture supported by the Microbiology Society
| 16.45 – 17.20 | Feng Shao, National Institute of Biological Sciences,  China. Hijacking the LPS-sensing noncanonical inflammasome pathway by bacterial effectors


| 17.20 – 17.50 | Poster mini talks 

Andreas Rummel, Hannover Medical School, Germany

Rachel Prescott. New York University Grossman School of Medicine

Sandra Sousa, Universidade do Porto, Portugal

Gunnar Schroeder, Queen’s University Belfast, UK

Shivneet Gill, University of Toronto, Canada
Juliane Bubeck Wardenburg, Washington University, USA


| 17.50 – 18.30 | Panel Discussion

| 19:00 | Dinner

| 20:30 - 22:30 | Posters and social

Day 3, Tuesday AM, June 27, 2023

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Session 3 - Divergent roles of bacterial effectors and toxins 

Chair: Vassilis Koronakis

| 9.00 – 9.30 | Bart Thomma, University of Cologne, Germany. Plant pathogens manipulate host microbiota to promote disease development

| 9.30 – 9.45 | Elena Kudryashova, Ohio State University, USA. Pointed-end processive elongation of actin filaments by Vibrio effectors VopF and VopL revealed by single-molecule imaging 

| 9.45 – 10.15 | Ethel Bayer-Santos, University of São Paulo, Brazil. The Salmonella T6SS arsenal to overcome colonization resistance

| 10.15 – 10.30 | David Albesa-Jové, University of the Basque Country, Spain. The Pseudomonas aeruginosa effector Tse5 forms membrane pores disrupting the membrane potential of intoxicated bacteria

| 10.30 – 10.45 | Poster mini talks 

Paweena Chaoprasid, University of Münster, Germany

Filippo Cattalani Tognola, University of Bern, Switzerland

Alexander Belyy, Max Planck Institute of Molecular Physiology, Germany

| 10.45 – 11:15 | Coffee Break

| 11.15 – 11.45Neal Alto,  University of Texas Southwestern Medical Center, USA. Mapping Effector Protein Functions at Single Cell Resolution

| 11.45 – 12.00 | Seema Mattoo, Purdue University, USA. Fic-mediated GMPylation: Discovery of a Bordetella Fic protein with guanylyltransferase activity

| 12.00 – 12.30 | Jeongmin Song, Cornell University, USA. Salmonella AB toxins: biology, pathogenesis, and neutralization

| 12.30 – 12.45 | Dorothea Höpfner, University Medical Center Hamburg-Eppendorf, Germany. The DNA-binding induced (de)AMPylation activity of a Coxiella burnetii Fic-enzyme targets Histone H3  
 
| 12.45 – 13.00 | Poster mini talks 

Stefanie Lietz, Ulm University Medical Center, Germany

Oleg Sitsel, Max-Planck Institute of Molecular Physiology, Germany

Michael Costello, University of California, USA

| 13:00 – 14:30 | Lunch

Day 3, Tuesday PM, June 27, 2023 

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Session 4 - Bacterial toxins against bacteria
Chair: Christoph Tang

Lecture supported by the National Biofilms Innovation Centre
| 14.30 - 15.00  |  Laurie Comstock, University of Chicago, USA. Mobile genetic elements and antagonism in the gut microbiota

| 15.00 – 15.15 | Benjamin Ross, Dartmouth College Geisel School of Medicine, USA. Defense against the Bacteroidales type VI secretion system is mediated by recombinase-associated acquired interbacterial defense orphan immunity genes
 
| 15.15 – 15.45 | Tim Blower, University of Durham, UK. Slowing down to get ahead: self-targeting toxins of Mycobacterium tuberculosis
 
| 15.45 – 16.00 | Poster mini talks 

John Manion, Harvard Medical School, USA

Pablo Ezequiel Valguarnera, Washington University, USA

Charles Ericson, ETH Zürich, Switzerland

| 16.00 – 16.30 | Coffee Break

| 16.30 – 17.00 | Marion Schuller, University of Oxford, UK. Molecular basis and cellular consequences of DNA base ADP-ribosylation by the bacterial toxin DarT

 
Keynote lecture supported by Microbiology Society
| 17.00 – 18.00 | Chris Hayes, University of California, USA. Licensed to kill: Post-translational activation of an antibacterial effector protein



| 19:00 | Dinner

| 20:30 - 22:30 | Posters and social

Day 4, Wednesday AM, June 28, 2023 

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Session 5 - Host-Pathogen Interactions
Chair: Joe Barbieri

| 9.00 – 9.30 | Suzana Salcedo, University of Lyon, France. Studying TIR effectors in the context of bacterial pathogenesis
 
| 9.30 – 10.00 | Jost Enninga, Institut Pasteur, France. Investigating intracellular niche formation in situ at molecular resolution of enteroinvasive bacterial pathogens

Lecture supported by the journal Molecular Microbiology
| 10.00 – 10.30 | Teresa Thurston, Imperial College London. Bacterial effectors and their many ways to modulate immune signaling

| 10.30 – 11:00 | Coffee Break

| 11.00 – 11.30 | Tamara O’Connor, Johns Hopkins University School of Medicine, USA. The bacterial pathogen Legionella hijacks host cell peroxisomes for replication vacuole membrane expansion and integrity

| 11.30 – 11.45Anthony Davidson, University of Cambridge, UK. Salmonella invasion is self-limiting due to effector-driven activation of N-WASP

| 11.45 – 12.15 | Carmen Buchreiser, Institut Pasteur, France. Transkingdom signalling via Legionella pneumophila small regulatory RNAs  and extracellular vesicles

| 12.15 – 13:00 | Lunch

Day 4, Wednesday PM, June 28, 2023

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| 13:00 – 18:00 | Choice of activities, or free time to explore the resort

| 19.00 – late | Conference Dinner and Scottish Ceilidh

Day 5, Thursday AM, June 29, 2023 

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Session 6 - New perspectives on bacterial toxins and effectors
Chairs: Peter Sebo and Daniel Humphreys

Session 6 is supported by the Biochemical Society

| 9.00 – 9.30 | Emmanuel Lemichez, Institut Pasteur, France. The cnf1 toxin encoding gene confers a competitive advantage for gut colonization by ExPEC 


| 9:30 – 9:45Rebecca McHugh, University of Glasgow, UK. Repurposing Aurodox as an Antivirulence Treatment for Shiga-toxin-Producing E. coli Infections.

| 9:45 – 10:00 | Nimrod Nachmias, Hebrew University of Jerusalem, Israel. Systematic Discovery of Antibacterial and Antifungal Bacterial Toxins 

| 10.00 – 10:30 | Coffee Break

| 10:30 – 10.45 | Shan Li, Huazhong Agricultural University, China. Pathogen hijacks programmed cell death signaling by arginine ADPR-deacylization of caspases

| 10:45 – 11.00 | Eric Oswald, Université de Toulouse, France. Super OMVs to deliver toxins and PAMPS and block autophagic flux of the host cell


Keynote lecture supported by the Biochemical Society
| 11:00 – 12.00 | Teresa Frisan

University of Umeå, Sweden. Tissue microenvironment and bacterial genotoxins: an intricated liaison


| 12.00 – CloseETOX 2025 announcement and Close from Organisers

| 12:30 – 13:30 | Lunch 

| 14.00Buses depart